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spheroid culture medium recombinant murine egf  (PeproTech)


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    PeproTech spheroid culture medium recombinant murine egf
    Spheroid Culture Medium Recombinant Murine Egf, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spheroid+culture+medium+recombinant+murine+egf/spheroid+culture+medium+recombinant+murine+egf/pmc09449601-343-59-68
    Average 90 stars, based on 1 article reviews
    spheroid culture medium recombinant murine egf - by Bioz Stars, 2026-09
    90/100 stars

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    Produced:

    Article Title: Store‐operated calcium entry controls innate and adaptive immune cell function in inflammatory bowel disease
    Article Snippet: Isolated intestinal crypts were counted, embedded in Matrigel (Corning, NY, USA; growth factor reduced, phenol red‐free, LDEV‐free) on ice, and seeded into 24‐well plates (500 crypts in 50 μl Matrigel per well). .. Matrigel was polymerized for 15 min at 37°C and overlaid with 500 μl of spheroid culture medium (50% L‐WRN‐CM2 [conditioned medium produced using the cell line L‐WRN CRL‐3276, ATCC, Manassas, USA]) in advanced DMEM/F‐12 supplemented with 20% fetal bovine serum, 2 mM L‐glutamine, 50 units/ml penicillin, and 50 μg/ml streptomycin (Merck Millipore, Burlington, USA) (and additional supplements specific for mouse and human [Mouse: 50 ng/ml recombinant murine EGF (Peprotech, Hamburg, Germany). .. Human: 50 ng/ml recombinant human EGF (Peprotech), 10 mM nicotinamide (Sigma, St. Louis, USA), 0.5 μM A83‐01, 10 μM SB202190, 10 nM human gastrin I] (all from Sigma)).

    Recombinant:

    Article Title: Store‐operated calcium entry controls innate and adaptive immune cell function in inflammatory bowel disease
    Article Snippet: Isolated intestinal crypts were counted, embedded in Matrigel (Corning, NY, USA; growth factor reduced, phenol red‐free, LDEV‐free) on ice, and seeded into 24‐well plates (500 crypts in 50 μl Matrigel per well). .. Matrigel was polymerized for 15 min at 37°C and overlaid with 500 μl of spheroid culture medium (50% L‐WRN‐CM2 [conditioned medium produced using the cell line L‐WRN CRL‐3276, ATCC, Manassas, USA]) in advanced DMEM/F‐12 supplemented with 20% fetal bovine serum, 2 mM L‐glutamine, 50 units/ml penicillin, and 50 μg/ml streptomycin (Merck Millipore, Burlington, USA) (and additional supplements specific for mouse and human [Mouse: 50 ng/ml recombinant murine EGF (Peprotech, Hamburg, Germany). .. Human: 50 ng/ml recombinant human EGF (Peprotech), 10 mM nicotinamide (Sigma, St. Louis, USA), 0.5 μM A83‐01, 10 μM SB202190, 10 nM human gastrin I] (all from Sigma)).



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    spheroid culture medium recombinant murine egf  (PeproTech)
    90
    PeproTech spheroid culture medium recombinant murine egf
    Spheroid Culture Medium Recombinant Murine Egf, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spheroid+culture+medium+recombinant+murine+egf/spheroid+culture+medium+recombinant+murine+egf/pmc09449601-343-59-68
    Average 90 stars, based on 1 article reviews
    spheroid culture medium recombinant murine egf - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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